梅花鹿体内脑肠肽的表达及细胞定位分析

doi:10.11843/j.issn.0366-6964.2017.01.019

畜牧兽医学报 ›› 2017, Vol. 48 ›› Issue (1): 157-165.doi: 10.11843/j.issn.0366-6964.2017.01.019

梅花鹿体内脑肠肽的表达及细胞定位分析

刘骄，张曼，金鑫，范燕茹，田巧珍，杨银凤^*

（内蒙古农业大学兽医学院农业部动物疾病临床诊疗技术重点实验室，呼和浩特 010018）

收稿日期:2016-07-12 出版日期:2017-01-23 发布日期:2017-01-23
通讯作者: 杨银凤，女，教授，博士生导师，主要从事动物解剖组织学和分子生物学研究，E-mail: julie1963@163.com,Tel: 0471-4310743
作者简介:刘骄(1990-)，男，内蒙古包头人，硕士生，主要从事动物解剖组织学及分子生物学研究，E-mail: 15248177358@163.com
基金资助:
内蒙古自然科学基金（2013MS0408）

Ghrelin Expression and Cell Location Analysis inside Sika Deer

LIU Jiao, ZHANG Man, JIN Xin, FAN Yan-ru, TIAN Qiao-zhen, YANG Yin-feng^*

(College of Veterinary, Inner Mongolia Agricultural University/Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Hohhot 010018, China)

Received:2016-07-12 Online:2017-01-23 Published:2017-01-23

摘要/Abstract

摘要：

为了明确脑肠肽Ghrelin（又名胃饥饿素）在梅花鹿体内的表达及定位，采用反转录－聚合酶链式反应（RT-PCR）、实时荧光定量PCR（RT-qPCR）和免疫组织化学技术检测了梅花鹿食管、瘤胃、网胃、瓣胃、皱胃、十二指肠、空肠、回肠、盲肠、结肠、肝、胰、甲状腺、垂体前叶、肺、肾、脾及卵巢等器官中Ghrelin mRNA的相对表达量和Ghrelin蛋白的定位分布情况。RT-qPCR结果显示：Ghrelin mRNA在上述器官中均有表达，其中皱胃内的表达量显著高于其他器官（P<0.05）；免疫组织化学染色结果表明Ghrelin蛋白在这些器官中均有表达，在消化管内的分布最为广泛。梅花鹿体内Ghrelin mRNA和Ghrelin蛋白的特定表达模式，揭示这一新型分子在梅花鹿的生长发育过程中可能存有广泛的生物学作用，尤其在胃肠道功能方面可能作为一种重要的调节方式而存在。

Abstract:

In order to specify the Ghrelin expression and localization inside sika deer, we adopted the reverse transcriptase-polymerase chain reaction (RT-PCR), real-time fluorescent quantitative PCR (RT-qPCR ) and immunohistochemical technique to test the relative expression quantity of Ghrelin mRNA and Ghrelin protein location distribution in the esophagus, rumen, reticulum, omasum, abomasum, duodenum, jejunum, ileum, caecum, colon, liver, pancreas, thyroid, anterior pituitary, lung, kidney, spleen, ovarian and other organs of the sika deer. RT-qPCR results showed: Ghrelin mRNA was expressed in these organs, and the expression quantity in the abomasum was significantly higher than that of other organs (P<0.05); Immunohistochemical staining result showed that Ghrelin protein was expressed in these organs, most widely distributed in the digestive tract. The specific expression pattern of Ghrelin mRNA and Ghrelin protein inside Sika deer revealed that the new molecule may have extensive biological effect in the growth and development process of sika deer, and existed as an important adjusting mode especially in the aspect of gastrointestinal function.

中图分类号:

S852.1

刘骄，张曼，金鑫，范燕茹，田巧珍，杨银凤. 梅花鹿体内脑肠肽的表达及细胞定位分析[J]. 畜牧兽医学报, 2017, 48(1): 157-165.

LIU Jiao, ZHANG Man, JIN Xin, FAN Yan-ru, TIAN Qiao-zhen, YANG Yin-feng. Ghrelin Expression and Cell Location Analysis inside Sika Deer[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(1): 157-165.

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梅花鹿体内脑肠肽的表达及细胞定位分析

Ghrelin Expression and Cell Location Analysis inside Sika Deer

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